anti human cd25 2a3 169tm  (fluidigm)

Journal: Cell Reports Medicine

Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells

doi: 10.1016/j.xcrm.2025.102311

Figure Lengend Snippet: Generation of BACH2 -KO humanized mice for HIV-1 infection (A) Strategy for generating BACH2 -edited humanized mice. (B) Human immune cell reconstitution and T cell development in mice engrafted with BACH2 -KO or Cas9 ctrl CD34 + cells. Blood samples were collected 10–11 weeks after transplantation. (C and D) Human T cell and macrophage development in mice engrafted with BACH2 -KO or Cas9 ctrl CD34 + cells. Spleen samples were collected 12 weeks after transplantation. The total naive (CD45RA + CCR7 + ), central memory (CD45RA − CCR7 + ), effector memory (CD45RA − CCR7 − ), and regulatory (CD25 + Foxp3 + ) CD4 + T cells as well as macrophages were determined by flow cytometry. (E and F) HIV-1 infection in mice engrafted with BACH2 -KO or Cas9 ctrl CD34 + cells. Plasma viral loads (E) and loss of CD4 + T cells (F) were determined at indicated time points. (G–I) Quantification of HIV-1 infection. The frequency of p24 + of the CD3 + CD8 − population was measured by flow cytometry. The frequency of HIV-1 DNA + cells was determined by qPCR. In (F), p values were calculated using the two-way ANOVA and Holm-Sidak multiple comparisons test. Other p values were calculated using the unpaired t test. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: Anti-human CD25 (IL-2R)-169Tm , Fluidigm , Cat# 3169003B; RRID: AB_2938861.

Techniques: Infection, Transplantation Assay, Flow Cytometry, Clinical Proteomics